妞干网这里只有精品_国产一区二区精品福利地址_在线观看星空传 ,欧洲人妻丰满AV无码久久不卡_国产在线精品二区

產(chǎn)品中心

大鼠鈉鉀氯協(xié)同轉(zhuǎn)運(yùn)蛋白1(NKCC1)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK0482

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Rat NKCC1(Na-K-Cl Cotransporter 1) ELISA Kit
Reactivity:	Rat
Alternative Names:	BSC; BSC2; KILQS; NKCC1; PPP1R141; SLC12A2
Assay Type:	Sandwich
Sensitivity:	0.14 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NKCC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NKCC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NKCC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NKCC1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Frontiers in Molecular Neuroscience

Impact of GABA and nutritional supplements on neurochemical biomarkers in autism: a PPA rodent model study

日期：2025-04-17 IF：3.5

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.185	2.081
10.00	1.502	1.398
5.00	1.136	1.032
2.50	0.872	0.768
1.25	0.557	0.453
0.63	0.326	0.222
0.32	0.226	0.122
0.00	0.104	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NKCC1 and the recovery rates were calculated by comparing the measured value to the expected amount of NKCC1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NKCC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.