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大鼠高敏型心肌肌鈣蛋白T(hs-cTnT)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK0844

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Rat hs-cTnT(High Sensitivity Cardiac Troponin T) ELISA Kit
Reactivity:	Rat
Alternative Names:	High Sensitivity Cardiac Troponin T
Assay Type:	Sandwich
Sensitivity:	5.2 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve.

Cellular and Molecular Biology

Impacts of trelagliptin and remogliflozin alone and in combination with Alpha Lipoic Acid on cardiac function in streptozotocin-induced diabetes mellitus in rats

日期：2023-10-08 IF：1.6
Naunyn-Schmiedeberg's Archives of Pharmacology

Cilostazol alleviates imatinib-induced myocardial injury in rats by modulating the TGF-β1/MAPK, SHC/Grb2/SOS signaling pathways and upregulating miRNA-195-5P

日期：2025-04-17 IF：3.1

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.075	1.992
500.00	1.630	1.547
250.00	1.116	1.033
125.00	0.815	0.732
62.50	0.539	0.456
31.25	0.313	0.230
15.63	0.252	0.169
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-cTnT and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnT in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	79-93%	86%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.