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8-羥基脫氧鳥苷(8-OHdG)酶聯(lián)免疫吸附檢測試劑盒

ELK10848

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
Reactivity:	General
Alternative Names:	8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Type:	Competitive Inhibition
Sensitivity:	0.57 ng/mL
Standard:	100 ng/mL
Detection Range:	1.56-100 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

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標準曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	0.221
50.00	0.411
25.00	0.692
12.50	0.913
6.25	1.225
3.13	1.587
1.57	1.813
0.00	2.325

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.

Matrix	Recovery range	Average
serum(n=5)	94-107%	100%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	94-105%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.