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斑馬魚類雌二醇(E2)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK10939

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Zebrafish E2(Estradiol) ELISA Kit
Reactivity:	Zebrafish
Alternative Names:	17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type:	Competitive Inhibition
Sensitivity:	12.46 pg/mL
Standard:	3000 pg/mL
Detection Range:	46.88-3000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Fish Physiology and Biochemistry

Role of a non-steroidal aromatase inhibitor on growth performance, body composition, reproductive and serum biochemical indices, and intestinal histomorphology in hybrid red tilapia (Oreochromis spp.)

日期：2025-03-17 IF：2.5

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
3000.00	0.199
1500.00	0.427
750.00	0.596
375.00	0.913
187.50	1.157
93.75	1.583
46.88	1.762
0.00	2.112

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-99%	92%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	87-99%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.