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小鼠髓樣前體細(xì)胞抑制因子2(MPIF2)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1235

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview

Product name:	Mouse MPIF2(Myeloid Progenitor Inhibitory Factor 2) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CCL24; SCYA24; Ckb-6; Eotaxin-2; Chemokine C-C-Motif Ligand 24; Eosinophil chemotactic protein 2; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 24
Assay Type:	Sandwich
Sensitivity:	6 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MPIF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MPIF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MPIF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MPIF2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.898	1.812
500.00	1.487	1.401
250.00	0.975	0.889
125.00	0.661	0.575
62.50	0.477	0.391
31.25	0.351	0.265
15.63	0.195	0.109
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MPIF2 and the recovery rates were calculated by comparing the measured value to the expected amount of MPIF2 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-105%	98%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	85-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MPIF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.