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大鼠谷氨酸半胱氨酸連接酶催化亞基(GCLC)酶聯(lián)免疫吸附檢測試劑盒

ELK6793

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Rat GCLC(Glutamate Cysteine Ligase, Catalytic) ELISA Kit
Reactivity:	Rat
Alternative Names:	GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS
Assay Type:	Sandwich
Sensitivity:	0.122 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;
Uniprot ID:	P19468
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GCLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GCLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GCLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GCLC in the samples is then determined by comparing the OD of the samples to the standard curve.

Immunopharmacology and Immunotoxicology

Reno-protective impact of diosmin and perindopril in amikacin-induced nephrotoxicity rat model: modulation of SIRT1/p53/C-FOS, NF-κB-p65, and keap-1/Nrf2/HO-1 signaling pathways

日期：2025-03-17 IF：2.9
Naunyn-Schmiedeberg's Archives of Pharmacology

Cilostazol alleviates imatinib-induced myocardial injury in rats by modulating the TGF-β1/MAPK, SHC/Grb2/SOS signaling pathways and upregulating miRNA-195-5P

日期：2025-04-17 IF：3.1

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.057	1.953
10.00	1.673	1.569
5.00	1.215	1.111
2.50	0.921	0.817
1.25	0.526	0.422
0.63	0.331	0.227
0.32	0.168	0.064
0.00	0.104	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GCLC and the recovery rates were calculated by comparing the measured value to the expected amount of GCLC in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	84-96%	90%
Heparin plasma(n=5)	80-95%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCLC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.