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大鼠興奮性氨基酸轉運蛋白2(EAAT2)酶聯(lián)免疫吸附檢測試劑盒

ELK7105

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Rat EAAT2(Excitatory Amino Acid Transporter 2) ELISA Kit
Reactivity:	Rat
Alternative Names:	SLC1A2; EAAT2; GLT-1; Solute Carrier Family 1 Member 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2
Assay Type:	Sandwich
Sensitivity:	0.056 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Neuro science;
Uniprot ID:	P31596
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EAAT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EAAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EAAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EAAT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cells

Venlafaxine and L-Thyroxine Treatment Combination: Impact on Metabolic and Synaptic Plasticity Changes in an Animal Model of Coexisting Depression and Hypothyroidism

日期：2021-06-05 IF：6.6
Frontiers in Molecular Neuroscience

Impact of GABA and nutritional supplements on neurochemical biomarkers in autism: a PPA rodent model study

日期：2025-04-17 IF：3.5

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.270	2.182
5.00	1.636	1.548
2.50	1.069	0.981
1.25	0.847	0.759
0.63	0.527	0.439
0.32	0.345	0.257
0.16	0.196	0.108
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant EAAT2 and the recovery rates were calculated by comparing the measured value to the expected amount of EAAT2 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	81-93%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EAAT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.