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大鼠維生素D3(VD3)酶聯(lián)免疫吸附檢測試劑盒

ELK8950

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Rat VD3(Vitamin D3) ELISA Kit
Reactivity:	Rat
Alternative Names:	Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
Assay Type:	Competitive Inhibition
Sensitivity:	2.11 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat VD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Frontiers in Molecular Neuroscience

Impact of GABA and nutritional supplements on neurochemical biomarkers in autism: a PPA rodent model study

日期：2025-04-17 IF：3.5

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
400.00	0.212
200.00	0.433
100.00	0.575
50.00	0.959
25.00	1.197
12.50	1.511
6.25	1.755
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VD3 and the recovery rates were calculated by comparing the measured value to the expected amount of VD3 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	80-95%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.