亚洲色资源在线播放_亚洲av不卡无码久久_国产中文字幕永久在线观看_日韩人妻在线视频二区 ,亚洲精品白浆高清久久久久久_久久久久久伊人高潮 ,香蕉免费一区二区三区在线观看,国产成人麻豆亚洲综合无码精

產(chǎn)品中心

人CD177分子(CD177)酶聯(lián)免疫吸附檢測試劑盒

ELK9330

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Human CD177(CD177 antigen) ELISA Kit
Reactivity:	Human
Alternative Names:	CD177; NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177
Assay Type:	Sandwich
Sensitivity:	28 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	Q8N6Q3
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD177. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD177. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD177, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD177 in the samples is then determined by comparing the OD of the samples to the standard curve.

MOLECULAR MEDICINE

Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury

日期：2025-03-17 IF：6

標準曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.096	1.994
2500.00	1.593	1.491
1250.00	1.186	1.084
625.00	0.871	0.769
312.50	0.527	0.425
156.25	0.262	0.160
78.13	0.256	0.154
0.00	0.102	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CD177 and the recovery rates were calculated by comparing the measured value to the expected amount of CD177 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	81-97%	89%
Heparin plasma(n=5)	83-99%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD177 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.